ABSTRACT
OBJECTIVE: To establish an HPCE method for determination of N-trans-feruloyl tyramine in Lycii Fructus. METHODS: The chromatographic separation was performed on an uncoated fused silica capillary of 50 cm × 75 μm ID (40 cm of effective length). 50 mmol · L-1Na2B4O7(pH 9.0 with H3PO4) was selected as the running buffer. The applied voltage was 20 kV, and the column temperature was maintained at 25°C. The chromatogram was detected at 214 nm. RESULTS: The linearity for N-trans-feruloyl tyramine was in a range of 0.25 - 15 μg · mL-1 (r = 0.9998, n = 7). The average recovery for N-trans-feruloyl tyramine was 101.8% with RSD of 2.06%. CONCLUSION: The method is accurate, reliable, and can be used as a quality control method for N-trans-feruloyl tyramine in Lycii Fructus, which can provide basis for development and utilization of Lycii Fructus. Copyright 2012 by the Chinese Pharmaceutical Association.